there was a dna analysis, if he were female it would have shown up there....
I think I have explained it a few posts earlier (post #32). The proposed explanation was:
he was born female, but his body was later heavily masculinized due to a condition known as hyperactivity of the adrenal gland
If you read the article, you would see they mentioned some mitochondrial dna analysis which revealed he was female (this was also supported by some characteristics of his/her bones).
The phenomenon is well known in medicine. The person affected was in the past more often referred to as hermaphrodite, today we tend to term such a person as an intersex person. Such a condition may have several reasons, one of them is attributed to the said hyperactivity of the adrenal gland which is responsible for the high levels of androgens at the early stage of human development (foetus). Nothing sensational about it really except for the fact that Pulaski is a well-known historical figure.
In legal terms, Kazimierz Pułaski will always remain a man and nothing is going to change that. Also, it is pretty certain that he considered himself male rather than female.